t-PSA
ZECEN
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PRINCIPLE OF THE TEST
Sandwich method：
t-PSA Antibody labeled by FITC and t-PSA antibody pair labeled by AP bind with the t-PSA antigen in the sample, control or calibrator and form sandwich complex. Then add the magnetic beads binding with Anti-FITC, through the specific binding between the FITC and Anti-FITC, the complex will be bound by the magnetic beads. Then the entire complex will be captured by the external applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through software by comparing the luminous intensity with the cutoff value to determine whether the corresponding antibody exists.
SPECIMEN COLLECTION AND PREPARATION
Sample material: serum
Collect samples using standard procedures.
Sample volume cannot be less than 200μL.
Store at 2-8℃: 24 hours, for longer storage periods: freeze to below - 20℃
Avoid repeated freezing and thawing cycles, stored samples should be thoroughly mixed prior to use (Vortex mixer).
PRINCIPLE OF THE TEST
Sandwich method：
t-PSA Antibody labeled by FITC and t-PSA antibody pair labeled by AP bind with the t-PSA antigen in the sample, control or calibrator and form sandwich complex. Then add the magnetic beads binding with Anti-FITC, through the specific binding between the FITC and Anti-FITC, the complex will be bound by the magnetic beads. Then the entire complex will be captured by the external applied magnetic field and separate from the unbound substance. After washing, add the substrate. The substrate will be catalytically cracked under the action of the enzyme, and form an unstable intermediate in excited state. When the intermediate in excited state returns to the ground state, it will issue photons and make a light-emitting reaction. Then the CLIA analyzer will measure the luminous intensity and count the results through software by comparing the luminous intensity with the cutoff value to determine whether the corresponding antibody exists.
SPECIMEN COLLECTION AND PREPARATION
Sample material: serum
Collect samples using standard procedures.
Sample volume cannot be less than 200μL.
Store at 2-8℃: 24 hours, for longer storage periods: freeze to below - 20℃
Avoid repeated freezing and thawing cycles, stored samples should be thoroughly mixed prior to use (Vortex mixer).
